MAB Purification Kit using Protein A Column and Desalting Column
Complete purification solution for monoclonal antibodies. This kit contains one Protein A column and one Desalting column, along with all necessary buffers, connectors, and a detailed protocol.
Key Features:
- Available in 3 scales – 25 mg, 50 mg, and 250 mg of MAB
- Compatible with bench-top chromatography systems, such as ÄKTA
- Works with manual, syringe-driven protocol
- Complete reagent set included
Kit Components
| Component | 25 mg MAB | 50 mg MAB | 250 mg MAB |
|---|---|---|---|
| Protein A Column | 0.5 mL | 1.0 mL | 5.0 mL |
| Desalting Column | 5 mL | 10 mL | 50 mL |
| Protein A Loading Buffer 10× | 30 mL | 30 mL | 100 mL |
| Protein A Elution Buffer 2× | 30 mL | 30 mL | 100 mL |
| Protein A Neutralization Buffer | 10 mL | 10 mL | 30 mL |
| Desalting Buffer 10× | 30 mL | 30 mL | 100 mL |
Resin Specifications
| Specification | Protein A | Desalting |
|---|---|---|
| Matrix | Cross-Linked Agarose | Cross-Linked Dextran |
| Bead Size | 50 µm | 20–80 µm |
| Capacity | Up to 70 mg/mL MAB | 20–25% of Bed Volume |
| Sample Dilution | N/A | 1.2 to 4-fold |
| pH Limit (Operational) | 3–12 | 3–12 |
| pH Limit (CIP) | 2–13 | 3–12 |